EDUCATION

University of Vermont College of Medicine- Burlington, VT

· MD, PhD student

University of Massachusetts Amherst- Amherst, MA

· BS Biology- Graduation May, 2001

Academic Awards/Achievements

· Recipient of a Howard Hughes Medical Institute Summer Student Research Fellowship (1998/1999/2000)

SCIENCE WORK

University of Vermont College of Medicine- Burlington, VT

· Graduate student (2003-present)

Harvard Medical School / Brigham and Women’s Hospital- Boston, MA

· HHMI Undergraduate Summer Research Student (1998/1999/2000)

· Research Assistant II (June, 2001- August, 2003)

Laboratory Techniques

· Techniques for analyzing DNA and RNA including isolation, cloning, southern blotting, northern blotting, PCR, rtPCR, retroviral transduction and other associated techniques

· Techniques for analyzing proteins including purification, western blotting, chromatography and other associated techniques

· Techniques for the use of mice in research including breeding, genotyping, necropsy, gavage, flow cytometry, bone-marrow transplantation and other associated techniques

· Certified to use radionuclides in research (Harvard University)

Laboratory Projects

· Further characterized a signaling complex and worked towards identifying a phospho-protein with possible importance in TEL/PDGFbR mediated leukemogenesis

· Evaluated the efficacy of specific kinase inhibitors in mouse bone-marrow transplant models of Leukemia

· Designed and constructed a conditional TEL/AML1 knock-in mouse, a Vav-Cre transgenic mouse and a Vav-Flt3 transgenic mouse

· Worked on cloning all point mutations in Flt3 previously seen in human AML and characterizing their sensitivity to Flt3 inhibitors in vitro

· Collaborated to identify novel Janus Kinase (Jak) family inhibitors

· Responsible for breeding/maintaining transgenic mouse lines

· Designed and implemented a method for creating a central DNA “plasmid bank” and created a corresponding database

PUBLICATIONS

· Lee BH, Williams IR, Anastasiadou E, Boulton CL, Joseph SW, Amaral SM, Curley DP, Duclos N, Huntly BJ, Fabbro D, Griffin JD, Gilliland DG. FLT3 internal tandem duplication mutations induce myeloproliferative or lymphoid disease in a transgenic mouse model. Oncogene. 2005 Aug 22; [Epub ahead of print]

· Growney JD, Shigematsu H, Li Z, Lee BH, Adelsperger J, Rowan R, Curley DP, Kutok JL, Akashi K, Williams IR, Speck NA, Gilliland DG. Loss of Runx1 perturbs adult hematopoiesis and is associated with a myeloproliferative phenotype. Blood. 2005 Jul 15;106(2):494-504

· Clark JJ, Cools J, Curley DP, Yu JC, Lokker NA, Giese NA, Gilliland DG. Variable sensitivity of FLT3 activation loop mutations to the small molecule tyrosine kinase inhibitor MLN518. Blood. 2004 Nov 1;104(9):2867-72

· Huntly BJ, Shigematsu H, Deguchi K, Lee BH, Mizuno S, Duclos N, Rowan R, Amaral S, Curley DP, Williams IR, Akashi K, Gilliland DG. MOZ-TIF2, but not BCR-ABL, confers properties of leukemic stem cells to committed murine hematopoietic progenitors. Cancer Cell. 2004 Dec;6(6):587-96.

· Cools J, Stover EH, Boulton CL, Gotlib J, Legare RD, Amaral SM, Curley DP, Duclos N, Rowan R, Kutok JL, Lee BH, Williams IR, Coutre SE, Stone RM, DeAngelo DJ, Marynen P, Manley PW, Meyer T, Fabbro D, Neuberg D, Weisberg E, Griffin JD, Gilliland DG. PKC412 overcomes resistance to imatinib in a murine model of FIP1L1-PDGFRalpha-induced myeloproliferative disease. Cancer Cell. 2003 May;3(5):459-69.

· Kelly LM, Yu JC, Boulton CL, Apatira M, Li J, Sullivan CM, Williams I, Amaral SM, Curley DP, Duclos N, Neuberg D, Scarborough RM, Pandey A, Hollenbach S, Abe K, Lokker NA, Gilliland DG, Giese NA. CT53518, a novel selective FLT3 antagonist for the treatment of acute myelogenous leukemia (AML). Cancer Cell. 2002 Jun;1(5):421-32.

· Sternberg DW, Tomasson MH, Carroll M, Curley DP, Barker G, Caprio M, Wilbanks A, Kazlauskas A, Gilliland DG. The TEL/PDGFbetaR fusion in chronic myelomonocytic leukemia signals through STAT5-dependent and STAT5-independent pathways.

· Kelly LM, Kutok JL, Williams IR, Boulton CL, Amaral SM, Curley DP, Ley TJ, Gilliland DG. PML/RARalpha and FLT3-ITD induce an APL-like disease in a mouse model. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8283-8.

ACKNOWLEDGMENTS FOR TECHNICAL SUPPORT

· Weisberg E, CL Boulton, LM Kelly, P Manley, D Fabbro, T Meyer, DG Gilliland, JD Griffin. Inhibition of mutant FLT3 receptors in leukemia cells by the small molecule tyrosine kinase inhibitor PKC412. Cancer Cell 2002 (online-5/31/01)

VOLUNTEER WORK

Cape Cod Hospital- Hyannis, MA (winter 1999/2000)

· Assisted with general patient care in the Intensive Care Unit and Cardiology

OTHER EMPLOYMENT

St. Francis Preparatory School- Hyannis, MA (winter 2000/2001)

· Substitute teacher (fourth through sixth grade levels)

Sturgis Charter High School- Hyannis, MA (winter 2000/2001)

· Substitute teacher (high school levels)

TEACHING/LEADERSHIP

Teaching Assistant- University of Massachusetts Amherst

· Responsible for teaching the laboratory portion of a human anatomy and physiology course (Biology 339)

Drum Major- University of Massachusetts Minuteman Marching Band

· Responsible for instructing, leading and conducting a 350 member marching band